BIOACCUMULATION OF IRON (Fe) IN Bacillus JA1, Sporosarcina JA4, AND Lysinibacillus JB2

Carbonatogenic bacteria are able to produce calcium carbonate (CaCO3). Steel reinforcement of concrete is mainly made a mixture carbon (C) and ferrous (Fe) elements. Fe classified as heavy metal, when in high concentrations it toxic bacteria. The purpose this study was determine the bioaccumulation carbonatogenic Fe. isolates used were Bacillus JA1, Sporosarcina JA4, Lysinibacillus JB2. All subcultured on nutrient agar slant media. Cultures minimal salt medium: broth (95% : 5%) with 24 hours incubation. Bioaccumulation test using containing 5 ppm 10 ppm. Accumulated concentration measured atomic absorption spectrophotometry. result research that by after 2 incubation greater than 1 hour JA1 has highest ability which 9,19 exposure its efficiency 91,85%.